کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1184015 | 1491808 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomics of human plasma: A critical comparison of analytical workflows in terms of effort, throughput and outcome
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
In this study, several workflows to analyze human plasma proteins with RP-LC MS/MS are evaluated. The impact of depletion of abundant proteins on the plasma proteome coverage was assessed together with the duration of RP-LC separation. An additional upstream liquid-based fractionation was evaluated. The applicability and feasibility of these technologies in large-scale clinical studies with respect to effort, throughput, and outcome are discussed. Label-free and isobaric tagging-based quantifications are examined in this perspective. We demonstrate that, despite the great improvement of proteomic technologies, significant trade-offs between effort and yield are still challenging the discovery of protein biomarkers in blood plasma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: EuPA Open Proteomics - Volume 1, 2013, Pages 8–16
Journal: EuPA Open Proteomics - Volume 1, 2013, Pages 8–16
نویسندگان
Loïc Dayon, Martin Kussmann,