کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212552 | 1494074 | 2014 | 8 صفحه PDF | دانلود رایگان |
• Alternative method for polishing albumin utilising HCIC.
• HCIC is more efficient and produces a higher purity product than current method.
• HCIC method does not require addition of lyotropic salts.
• HCIC method can be easily incorporated into existing plasma fractionation processes.
The manufacture of human serum albumin by chromatographic procedures involves gel filtration chromatography as a final polishing step. Despite this step being essential to remove high molecular weight impurity proteins and thus ensure a stable and safe final product, it is relatively inefficient. This paper explores the use of hydrophobic charge induction chromatographic media, MEP HyperCel as an alternative to Sephacryl S200HR gel filtration for the polishing of human serum albumin derived by ion exchange chromatographic purification of Cohn Supernatant I. The use of MEP HyperCel results in a product with a higher purity than achieved with gel filtration and in a less time consuming manner and with potential resource savings. MEP HyperCel appears to have great potential for incorporation into downstream processes in the plasma fractionation industry as an efficient means of achieving polishing of intermediates or capture of proteins of interest.
Journal: Journal of Chromatography B - Volume 969, 15 October 2014, Pages 241–248