کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1222600 | 967868 | 2011 | 6 صفحه PDF | دانلود رایگان |
The current study demonstrates the reversal of enantiomer migration order (EMO) in capillary electrophoresis (CE) based separations of sibutramines (SIB) as a function of the concentration of two types of cyclodextrin (CD), native β-CD and acetyl-β-CD. At normal working concentrations (<10 mM) of either CD, (S)-SIB migrated first. However, at CD concentrations greater than 10 mM, (R)-SIB was the first to migrate. This study describes factors involved in determining EMO for sibutramine enantiomers at low and high concentrations of CDs. The reversal of EMO could be explained in terms of the opposing effects of the stability and the limiting complex mobility of the SIB-CD complexes. The enantioseparation of SIB with methyl- and 2-hydroxypropyl-β-CD was possible based on differences in the binding constants of complexes. However, reverse EMO was not observed because of equal mobilities of SIB enantiomers complexed with methyl- and 2-hydroxypropyl-β-CD.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 54, Issue 5, 5 April 2011, Pages 1007–1012