Carbohydrate transporting membrane proteins of the rumen bacterium, Butyrivibrio proteoclasticus

The research was aimed at finding which membrane proteins of the rumen bacterium Butyrivibrio proteoclasticus are involved in the uptake of carbohydrates resulting from extracellular enzymatic degradation of hemicellulose and fructan. The proteomic analysis of cells grown with fructose or xylan as the sole substrate identified 13 membrane proteins predicted to function as carbohydrate transporters. One protein detected was the membrane component of a fructose-specific phosphoenolpyruvate:sugar phosphotransferase system believed to be involved in the fructose uptake following extracellular fructan breakdown. The other 12 proteins were all ABC transport system substrate-binding proteins, nine of which belong to functional category COG1653 that includes proteins predicted to transport oligosaccharides. Four of the SBPs were significantly upregulated in xylan grown cells, and three of these were found in polysaccharide utilisation loci where they are clustered with other genes involved in hemicellulose breakdown and metabolism. It is possible that the carbon source available regulates a wider network of genes. The information on the mechanisms used by rumen bacteria to take up carbohydrates from their environment may improve our understanding of the ruminant digestion and facilitate strategies for improved pasture and stored feed utilisation.

A model of fructose and hemicellulose transport by membrane proteins in rumen bacteria Butyrivibrio proteoclasticus.Figure optionsDownload high-quality image (102 K)Download as PowerPoint slideHighlights
► The bacterium Butyrivibrio proteoclasticus metabolises plant cell walls.
► A model of carbohydrate uptake following extracellular digestion is supported.
► Membrane proteins involved in carbohydrate transport were identified.
► ABC transport system proteins were up-regulated in xylan grown cells.
► There are implications for rumen metabolism and ruminant production.