کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1390402 | 1500901 | 2012 | 9 صفحه PDF | دانلود رایگان |
Using 60% (w/v) ammonium sulfate precipitation, a heteropolysaccharide (designated 60S), with relatively low molecular weight (38.7 kDa), was isolated from the seeds of Artemisiasphaerocephala Krasch. The structural properties of 60S were elucidated by partial acid hydrolysis, methylation analysis, 1D and 2D NMR spectroscopy, and MALDI-TOF-MS. The results of the partial acid hydrolysis and methylation analysis indicated that the main chain of 60S consisted of (1→4)-linked d-Manp and (1→4)-linked d-Glcp in a molar ratio of 1:1.3. Over half of the glucosyl residues in the main chain were branched at the O-6 position. The terminal sugar residues were mainly composed of T-Araf, T-Arap, T-Galp, T-GlcpA, and T-Glcp. Besides, 3-Araf and 2-Galp were also observed in comparable amounts. Based on all the aforementioned results and the data obtained by 1D and 2D NMR spectroscopy as well as MALDI-TOF-MS, a structure of 60S is proposed as follows:R could be one or some of -(3-α-Araf)n-(A), T-α-Galp (B), T-α-Glcp (C), T-Araf (H) or T-Arap.
Figure optionsDownload as PowerPoint slide
Journal: Carbohydrate Research - Volume 350, 1 March 2012, Pages 31–39