کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1589432 1001991 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Histochemical and ultrastructural changes of sternomastoid muscle in aged wistar rats
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد دانش مواد (عمومی)
پیش نمایش صفحه اول مقاله
Histochemical and ultrastructural changes of sternomastoid muscle in aged wistar rats
چکیده انگلیسی

The aim of this study was to evaluate histochemically and ultrastructurally the sternomastoid muscle (SM) of adults and aged rats, employing histochemic (NADH-TR reaction) and transmission electron microscopic methods. It was used 20 rats, divided into two groups: adults (n = 10), animals with 4 months of age, and aged group (n = 10), animals with 24 months of age. Five animals from each group were anesthetized with an overdose of urethane (3 g/kg i.p.), and the muscles dissected after the samples processing for histochemical reaction (NADH-TR). Three types of fibers were identified by their metabolic characteristics: fibers with high oxidative capacity (O), intermediate oxidative capacity (OG) and low oxidative capacity (G). For transmission electron microscopic method, the animals were anesthetized and perfused by modified Karnovsky solution and the tissues were postfixed in 1% osmium tetroxide solution, dehydrated and embedded in Spurr resin. It was performed ultra-thin sections for transmission electron microscopic analysis. The SM showed heterogeneity in their composition according to the fiber types, with significant difference (p < 0.05) when comparing the fibers types between the superficial and deep regions and between the adult and aged groups. It was observe a decrease between the comparison of the total fibers density and GO fiber, and an increase of the O fiber in aged group. Ultrastructural characteristics of muscle cells in aged group showed typical morphological changes, characterizing muscular atrophy. We conclude based on physiological ageing process, changes in muscle fibers classification, and ultrastructuraly, morphological alterations on muscle cells, characterizing a muscular atrophy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Micron - Volume 42, Issue 8, December 2011, Pages 871–876
نویسندگان
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