کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1589523 | 1001997 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Imaging label-free intracellular structures by localisation microscopy Imaging label-free intracellular structures by localisation microscopy](/preview/png/1589523.png)
Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe–Rayleigh limit of about 200 nm laterally and 600 nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the λ/10 range (∼50 nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions.
Journal: Micron - Volume 42, Issue 4, June 2011, Pages 348–352