کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1589537 | 1001999 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Confocal fluorescence polarization microscopy for linear unmixing of spectrally similar labels Confocal fluorescence polarization microscopy for linear unmixing of spectrally similar labels](/preview/png/1589537.png)
Studies of biological samples often call for simultaneous identification of multiple molecular or structural components. Multiple labelling fluorescence techniques are a powerful way of achieving this. However, the ability to distinguish a number of fluorescent probes unambiguously can be restricted by the fact that fluorescence spectra are generally broad and overlapping. Recently a technique known as linear unmixing has been combined with spectral imaging to discriminate between multiple fluorophores. In this study a scheme is proposed whereby fluorescence polarization information is used to expand the capability of the linear unmixing technique to accommodate additional fluorescent probes. As a proof-of-concept, it is shown that this polarization-based technique can be used to divide the signals generated by two spectrally similar fluorescent probes into their separate components.
Journal: Micron - Volume 40, Issue 2, February 2009, Pages 212–217