Salivary system in leaf-cutting ants (Atta sexdens rubropilosa Forel, 1908) castes: A confocal study

The salivary system in ants is not only limited to digestory functions, but also has important role in the communication. The glands which compose the salivary complex are: the post-pharyngeal, hypopharyngeal, mandibular, and thoracic salivary gland, showing peculiar features which may vary according to the castes the individuals belong to and according to the functions they develop. The present study compared the morphological differences among the glands of Atta sexdens rubropilosa workers, males and queens focused on the organization of microfilaments and microtubules in these ants salivary system. Although the post-pharyngeal gland appeared to be more developed in queens, there were no significant gland differences among the analyzed castes. In what regards to the secretory units of the hypopharyngeal and mandibular glands, the association of F-actin with the collector duct seemed to be strong, being surrounded by a microtubules arrangement. The use of a laser scanning confocal microscopy with immunofluorescence whole mounting preparations revealed itself an efficient instrument for the understanding of the internal morphology of insects.