Characterization and antibacterial performance of ZrNO–Ag coatings

Composite metal nitride–metal (MeN–Me) physical vapor deposited (PVD) films (e.g. TaN–Cu, TiN–Cu, ZrN–Ag, etc.) are often considered for applications such as tribological and antibacterial coatings. In this study, ZrN, ZrO2–Ag and ZrNO–Ag coatings with different Ag contents were deposited by reactive magnetron sputtering. Monoclinic ZrO2 and fcc Ag composite structures were obtained when Zr and Ag were cosputtered to react with oxygen. ZrNO–Ag showed a porous structure containing monoclinic ZrO2, fcc Ag and Zr2ON2. Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and Staphylococcus aureus (S. aureus), major pathogens frequently found in the implant-associated infections, were cultured on the coated samples. The antibacterial effects were determined using in vitro anti-bacterial analyses by a fluorescence staining method employing Syto9 and bacterial viability agar tests. In addition, the biocompatibility of human gingival fibroblast (HGF) cells on coatings was also evaluated by a MTT test assay. It showed that the nanostructure and Ag content of the ZrO2–Ag and ZrNO–Ag coatings were correlated with the biocidal property. The ZrN coating deposited by magnetron sputtering did not improve the bacterial reduction of A. actinomycetemcomitans and S. aureus. The ZrO2–Ag and ZrNO–Ag coated samples had lower bacterial retention, and the ZrNO–Ag coatings with silver content of 11.8 at.% possessed the best antibacterial performance with excellent HGF cell compatibility as well.

► ZrN, ZrO2–Ag and ZrNO–Ag coatings were deposited by reactive magnetron sputtering.
► Monoclinic ZrO2 and fcc Ag composite structures were obtained for the deposited ZrO2–Ag.
► ZrNO–Ag showed a porous structure containing monoclinic ZrO2, fcc Ag and Zr2ON2.
► The ZrO2–Ag and ZrNO–Ag had lower retention of A. actinomycetemcomitans and S. aureus.
► ZrNO–Ag (Ag = 11.8 at.%) had the best antibacterial performance with good cell compatibility.