کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
16906 42621 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Effect of spermidine and its metabolites on the activity of pea seedlings diamine oxidase and the problems of biosensing of biogenic amines with this enzyme
ترجمه فارسی عنوان
اثر اسپرمیدین و متابولیت های آن بر فعالیت دیامین اکسیداز نهال نخود و مشکلات سنجش زیستی آمین های بیوژنیکی با این آنزیم
کلمات کلیدی
دیامین اکسیداز؛ نهال نخود؛ آمین biogenic اسپرمیدین؛ 1،3-دیامینو پروپان؛ بیوسنسور
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Spermidine in samples influences biosensing of other biogenic amines with PSAO.
• Spermidine acts as an inhibiting substrate to PSAO.
• Spermidine’s metabolite 1,3-diaminopropane acts as a competitive inhibitor.
• The inhibition of PSAO causes underestimation of biosensing results.
• The half-life of spermidine at room temperature is seven days.

Spermidine is one of the several biogenic amines, produced during the microbial decarboxylation of proteins. Individual biogenic amines in the formed mixtures are frequently analyzed with oxygen sensor based biosensors, as their content serves as a good biomarker for the determination of food quality. In these biosensors, diamine oxidase from pea seedlings (PSAO), catalyzing the oxidation of various biogenic amines by dissolved oxygen is commonly used for the bio-recognition of amines. However, in the presence of spermidine and/or its metabolite 1,3-diaminopropane, the activity of PSAO and the sensitivity of PSAO-based biosensors decrease due to inhibition. The inhibition constant of soluble spermidine, acting as an inhibiting substrate toward PSAO, was found to be (40 ± 15) mM in freshly prepared solution and (0.28 ± 0.05) mM in solution, incubated 30 days at room temperature. The inhibition constant of 1,3-diaminopropane, acting as a competitive inhibitor, was (0.43 ± 0.12) mM as determined through the oxidation reaction of cadaverine. The metabolic half-life of soluble spermidine was 7 days at room temperature and 186 days at 4 °C. The kinetic measurements were carried out with an oxygen sensor; the composition of the solution of degraded spermidine was analyzed with MS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 133–137
نویسندگان
, , , , ,