کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1931855 | 1050566 | 2010 | 8 صفحه PDF | دانلود رایگان |
ObjectivesTo determine the role of macrophage ATP-binding cassette transporter A5 (ABCA5) in cellular cholesterol homeostasis and atherosclerotic lesion development.Methods and resultsChimeras with dysfunctional macrophage ABCA5 (ABCA5−M/−M) were generated by transplantation of bone marrow from ABCA5 knockout (ABCA5−/−) mice into irradiated LDLr−/− mice. In vitro, bone marrow-derived macrophages from ABCA5−M/−M chimeras exhibited a 29% (P < 0.001) decrease in cholesterol efflux to HDL, whereas a 21% (P = 0.07) increase in cholesterol efflux to apoA-I was observed. Interestingly, expression of ABCA1, but not ABCG1, was up-regulated in absence of functional ABCA5 in macrophages. To induce atherosclerosis, the transplanted LDLr−/− mice were fed a high-cholesterol Western-type diet (WTD) for 6, 10, or 18 weeks, allowing analysis of effects on initial as well as advanced lesion development. Atherosclerosis development was not affected in male ABCA5−M/−M chimeras after 6, 10, and 18 weeks WTD feeding. However, female ABCA5−M/−M chimeras did develop significantly (P < 0.05) larger aortic root lesions as compared with female controls after 6 and 10 weeks WTD feeding.ConclusionsABCA5 influences macrophage cholesterol efflux, and selective disruption of ABCA5 in macrophages leads to increased atherosclerotic lesion development in female LDLr−/− mice.
Journal: Biochemical and Biophysical Research Communications - Volume 395, Issue 3, 7 May 2010, Pages 387–394