کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1938109 | 1050732 | 2007 | 6 صفحه PDF | دانلود رایگان |
The mRNA concentration of CAPN10, a T2D susceptibility gene was measured in white blood cells of T2D and healthy subjects, as well as the transcript half-life in two SNP-43 genotyped human cell lines, to evaluate a possible relationship between this SNP-43 and the transcript half-life. T2D patients with the SNP-43 G-allele had 4.6-fold more CAPN10 transcripts compared to subjects with the A-allele. The mRNA half-life of this transcript in 293T cells (SNP-43 G/G) and Jurkat cells (SNP-43 A/A) was of 8 h. We provide evidence that in T2D subjects the G-allele increases the CAPN10 mRNA levels. We propose a defective CAPN10 pre-mRNA processing is responsible for the decreased levels of SNP-43 A-allele transcripts in peripheral white cells of healthy and T2D individuals.
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 3, 6 July 2007, Pages 831–836