Does fluctuating thermal regime trigger free amino acid production in the parasitic wasp Aphidius colemani (Hymenoptera: Aphidiinae)?

When stressful cold-exposure is interrupted by short warm intervals, physiological recovery is possible, and this improves markedly the survival of insects. Fluctuating thermal regime (FTR) may act as a cue triggering the initiation of a metabolic response involving synthesis of cryoprotective compounds, such as free amino acids (FAA). Since specific changes in FAA levels can provide a good indication of the overall response of an organism to stressful conditions, we investigated temporal changes in FAA body contents of the parasitoid Aphidius colemani Viereck during exposure to FTR (4 °C: 20 °C for 22 h: 2 h per day) versus constant low temperature (4 °C). Physiological response during cold-exposure was clearly dissimilar between thermal treatments. Under constant cold-exposure FAA pool increased, whereas it decreased with cold-exposure duration in FTR. No single FAA accumulation could explain the higher survival under FTR. We propose that instead of considering FAA as a part of cryoprotective arsenal, FAA accumulation should rather be regarded as a symptom of a cold-induced physiological response. This is much less manifest under FTR, as the warm intervals likely allow a periodic reactivation of normal metabolic activities and a recovery of developmental processes.