کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2131987 | 1086668 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding of nuclear caveolin-1 to promoter elements of growth-associated genes in ovarian carcinoma cells
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کلمات کلیدی
mAbTX-100Cyclin D1ChrFCSDAPIGFPGSA4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولSDS–PAGE - SDS-PAGEGel shift assay - آزمون تغییر ژلMonoclonal antibody - آنتی بادی مونوکلونالchromatin immunoprecipitation - ایمن سازی کروماتینTriton X-100 - تریتون X-100 Gene transcription - رونویسی ژنOvarian carcinoma - سرطان تخمدانfetal calf serum - سرم گوساله جنینSol - سلendoplasmic reticulum - شبکه آندوپلاسمی Matrix - ماتریکسMeS - مسMolecular weight - وزن مولکولیgreen fluorescent protein - پروتئین فلورسنت سبزCHiP - چیپcaveolin-1 - کائولین-1Cav-1 - کاو-1Folate receptor - گیرنده Folate
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Caveolin-1 (cav-1), a member of a protein family associated mainly with cell membrane microdomains in many cell types, acts as a tumor suppressor in ovarian carcinoma cells. Biochemical analyses demonstrated that cav-1 was also localized in the nuclei of ovarian carcinoma cells, endogenously (SKOV3) or ectopically (IGtC3) expressing cav-1. By confocal analyses, the same cell lines as well as IGROV1 and SKOV3 cells transiently transfected with green fluorescent protein-cav-1 fusion protein showed nuclear punctate speckled pattern. Subnuclear distribution analysis revealed cav-1 mainly associated with the nuclear matrix, but also slightly with chromatin. Cav-1 was found in nuclear high-molecular weight complexes and by confocal analysis was found to co-localized with the inner nuclear membrane protein emerin. Cyclin D1 and folate receptor promoters were modulated by cav-1 in SKOV3 cells as demonstrated by transient transfection with or silencing of cav-1. Chromatin immunoprecipitation and supershift assays indicated that nuclear cav-1 can bind in vitro and in vivo to promoter sequences of both cyclin D1 and folate receptor genes. These data suggest that in ovarian carcinoma cells cav-1, localized in transcriptionally inactive chromatin, exerts a functional activity mediated, at least in part, by directly binding to sequences of genes involved in proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 313, Issue 7, 15 April 2007, Pages 1307-1317
Journal: Experimental Cell Research - Volume 313, Issue 7, 15 April 2007, Pages 1307-1317
نویسندگان
Elena Sanna, Silvia Miotti, Mimma Mazzi, Giuseppina De Santis, Silvana Canevari, Antonella Tomassetti,