کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
214998 1426215 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calorimetric investigation on the interaction of proflavine with human telomeric G-quadruplex DNA
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Calorimetric investigation on the interaction of proflavine with human telomeric G-quadruplex DNA
چکیده انگلیسی


• Energetics of the interaction of proflavine with G-quadruplex DNA is presented.
• The binding was exothermic and driven by negative enthalpy and positive entropy.
• The binding was characterized by enthalpy–entropy compensation.
• DSC studies revealed that proflavine stabilized the quadruplex significantly.

The interaction of an acridine dye, proflavine, with human telomeric G-quadruplex DNA was characterized by isothermal titration calorimetry and differential scanning calorimetry. The equilibrium constant of binding was deduced to be (1.36 ± 0.09) · 106 M−1 at T = 298.15 K. The binding reaction was driven by both negative enthalpy and positive entropy contributions. The equilibrium constant decreased and the reaction became increasingly enthalpy driven with rise in temperature. However, the standard molar Gibbs energy change exhibited only marginal alterations suggesting the occurrence of enthalpy–entropy compensation. Negative heat capacity values were also obtained from the temperature dependence of enthalpy change. Parsing of the standard molar Gibbs energy using the salt dependent calorimetric data revealed that the binding was dominated by non-polyelectrolytic forces which remained virtually unaltered with changing salt concentration. Proflavine binding also significantly enhanced the thermal stability of G-quadruplex DNA against thermal unfolding.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Chemical Thermodynamics - Volume 98, July 2016, Pages 208–213
نویسندگان
, ,