کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2169281 | 1092933 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Positive effects of cryopreserved adult or fetal liver cell transplants on hypercholesterolemia and hepatic antioxidant defenses in cholesterol-fed rabbits
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کلمات کلیدی
Hepatocytes - hepatocytesnon-alcoholic fatty liver disease - بیماری کبدی چربی غیر الکلیRabbit - خرگوشCryopreservation - سرماداری hepatic progenitor cells - سلول های پیش گیاه کبدیFetal liver cells - سلول های کبدی جنینLipoproteins - لیپوپروتئین هاLipid peroxidation - پراکسیداسیون لیپیدAllogenic transplantation - پیوند آلوژنیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
The liver plays a central role in lipid metabolism and the pathophysiology of many lipid disorders leads in turn to liver cell injury. Adult hepatocyte transplants provide well-recognized metabolic support, whilst hepatic stem cells may promote liver regeneration and repair, but in both cases, any clinical application would require low temperature banking of the cells. A model of dietary hypercholesterolemia was established in rabbits over 5 months, and transplants of cryopreserved adult hepatocytes (CH) and cryopreserved fetal liver cells (CFLC) were compared to Sham transplants. Cryopreservation was performed by a two-step freezing protocol using 1.5 mol/l dimethyl sulfoxide (Me2SO). Serum contents of cholesterol lipid classes were measured during the subsequent 4 weeks, in addition to markers of serum and liver oxidative stress. Both CH and CFLC transplantation resulted in a decrease of serum lipids during the 1st week after transplantation. The effect of CH was limited to the 1st week, but CFLC provided a sustained lipid-lowering effect over the 4 weeks. The ultimate outcome of CFLC transplantation by the end of 4 weeks was more pronounced and statistically significant for both serum total cholesterol (0.15 ± 0.05 versus 3.65 ± 1.4 mmol/l) and high-density lipoprotein-cholesterol (0.04 ± 0.01 versus 0.56 ± 0.06 mmol/l) compared to Sham transplants (p < 0.05 in both cases). CFLC transplantation also normalized hepatic tissue antioxidant defenses, namely an increase in reduced glutathione content, and enzyme activities for catalase and glutathione reductase (all significantly higher at p < 0.05 than in Sham transplants) by 4 weeks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 55, Issue 1, August 2007, Pages 72-79
Journal: Cryobiology - Volume 55, Issue 1, August 2007, Pages 72-79
نویسندگان
Alexander S. Lebedinsky, Daria V. Cherkashina, Alexander N. Sukach, Nataliya A. Volkova, Barry J. Fuller, Alexander Yu. Petrenko,