کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2197168 | 1550952 | 2009 | 11 صفحه PDF | دانلود رایگان |
A QPCR analysis of androgen receptor and several androgen metabolizing genes was performed during the saccular and alveolar stages of mouse lung development. Androgen receptor expression showed a statistically significant increase during the alveolar stage while levels of 17β-hydroxysteroid dehydrogenase type 2 (17β-HSD 2) expression significantly decreased at the end of the saccular stage and remained low throughout the alveolar period. 17β-HSD 1, 17β-HSD 5, 5α-reductase type 1, and mouse 3α-HSD did not present such a regulation. The androgen receptor protein was primarily detected in the nucleus of airway epithelial cells and of a subset of respiratory epithelial cells. 17β-HSD 2 mRNA co-localized with androgen receptor protein during saccularization, but was absent from airway epithelium during alveolarization. Taken together, our results demonstrate temporal and spatial regulation of androgen receptor and 17β-HSD 2 during the sacculo-alveolar transition period of mouse lung development suggesting control of androgen action.
Journal: Molecular and Cellular Endocrinology - Volume 311, Issues 1–2, 13 November 2009, Pages 109–119