کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2601046 | 1133295 | 2009 | 10 صفحه PDF | دانلود رایگان |
The effects of deoxynivalenol (DON) on the mRNA expression of cytokines and inflammation-related genes, as well as the cytokine secretion of porcine hepatocytes and Kupffer cell enriched hepatocyte cultures (co-cultures), were investigated in the absence or presence of LPS.DON and LPS acted in a synergistic manner with regard to a significantly increased mRNA expression of TNF-α in hepatocytes exposed to 500 nM or 2000 nM DON, or non-significant increase in co-cultures after 3 h of exposure. TNF-α supernatant concentrations were increased due to LPS but did not reflect the synergistic effects with DON as observed at mRNA level. IL-6 mRNA in hepatocyte cultures at 6 h paralleled the TNF-α supernatant pattern at this time point. In co-cultures and hepatocytes, a DON dose dependent induction of IL-6 mRNA was detected in cells not exposed to LPS. Supernatant concentrations of LPS-induced IL-6 were significantly decreased by 2000 nM DON in both types of cell cultures. Also the mRNA expression of the anti-inflammatory IL-10 was increased by DON to various degrees depending on DON-dose, stimulation with LPS and time point of measurement. After 6 h, expression of iNOS was only induced by 2000 nM DON, but not in LPS treated cells.Even if mRNA induction was not paralleled by related supernatant concentrations of TNF-α, IL-6 and IL-10 under the conditions of the present investigations, it was clearly demonstrated that DON has the potential to provoke and modulate immunological reactions of porcine liver cells.
Journal: Toxicology Letters - Volume 190, Issue 1, 8 October 2009, Pages 96–105