کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2603108 | 1133808 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pyrogallol inhibits the growth of human lung cancer Calu-6 cells via arresting the cell cycle arrest
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کلمات کلیدی
CDKCDKINADPHPyrogallolFBSXanthine oxidase - زانتین اکسیداز3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideMTT - MTTROS - ROSdihydroethidium - دی هیدروتیدیمSOD - سدfetal bovine serum - سرم جنین گاوSuperoxide dismutase - سوکسوکس دیسموتازcyclin-dependent kinase inhibitor - مهار کننده کیناز وابسته به سیکلینDHE - وPropidium iodide - پروتئین یدیدCell cycle - چرخه سلولیCatalase - کاتالازcyclin-dependent kinase - کییناز وابسته به سیکلینReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pyrogallol (PG) is a polyphenol compound and has been known to be an O2- generator. We evaluated the effects of PG on the growth of human pulmonary adenocarcinoma Calu-6 cells in relation to the cell cycle. DNA flow cytometric analysis indicated that PG induced a G2 phase arrest of the cell cycle in Calu-6 cells at 72Â h. PG down-regulated the expression of CDKI (p27), CDK2, CDK4 and CDK6 as well as cyclin D1, and increased cyclin A and cyclin B1 proteins. In addition, O2- levels were significantly increased in PG-treated cells. Treatment with catalase rescued Calu-6 cells from PG-induced apoptosis, and also prevented the growth inhibition as well as a G2 phase arrest by PG, which were accompanied with the down-regulation of O2- levels. In conclusion, PG inhibited the growth of Calu-6 cells by inducing the cell cycle arrest, accompanied with an increase in O2- levels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 22, Issue 6, September 2008, Pages 1605-1609
Journal: Toxicology in Vitro - Volume 22, Issue 6, September 2008, Pages 1605-1609
نویسندگان
Yong Hwan Han, Sung Zoo Kim, Suhn Hee Kim, Woo Hyun Park,