کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2603271 | 1133815 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The mycotoxin patulin, modulates tight junctions in caco-2 cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The mycotoxin patulin is a common contaminant of fruit. Here, we demonstrate that patulin reduces the barrier properties of the intestinal cell line, caco-2 by specific effects on tight junction components. Within 5 h of exposure to 100 μM toxin, the transepithelial electrical resistance of caco-2 monolayers was reduced by approximately 95% and the monolayer became more permeable to FITC-labelled dextrans of 4–40 kDa. Immunoblotting revealed occludin proteolysis and a significant reduction in ZO-1 levels. Patulin had no influence on claudin levels but marked changes in their distribution were observed. These data indicate that patulin decreases the barrier properties of caco-2 monolayers by modulation of the tight junction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 23, Issue 1, February 2009, Pages 83–89
Journal: Toxicology in Vitro - Volume 23, Issue 1, February 2009, Pages 83–89
نویسندگان
John McLaughlin, Daniel Lambert, Philip J. Padfield, Julian P.H. Burt, Catherine A. O’Neill,