کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2800177 | 1568901 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
OB-RL silencing inhibits the thermoregulatory ability of Great Roundleaf Bats (Hipposideros armiger)
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کلمات کلیدی
VMHBATshRNARNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsmall hairpin RNA - RNA کوچک موی سرelectrocardiograph - الکتروکاردیوگرافیECG - الکتروکاردیوگرام یا نوار قلبthird ventricle - بطن سومBody temperature - دمای بدنAmbient temperature - دمای محیطbeats per minute - ضربان در دقیقهArc - قوسBMP - مدیریت فرایند کسب و کارVentromedial hypothalamic nucleus - هسته هیپوتالاموس Ventromedialarcuate hypothalamic nucleus - هسته هیپوتالاموس قوس الکتریکیCdS - کادمیم سولفید، سولفید کادمیمLeptin receptors - گیرنده های لپتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous studies have shown that the hormone Leptin has an important role in mammalian heterothermy by regulating metabolism and food intake via lipolysis, as well as adaptive evolution of Leptin in heterothermic bats driven by selected pressure. However, the mechanism of Leptin in heterothermic regulation in mammals is unknown. By combining previous results, we speculated that the Leptin signaling pathway mediated by OB-RL (Leptin receptor long form) in the hypothalamus is important. OB-RL is one of the products of db gene and mainly distributed in the hypothalamus. In this study, we used OB-RL as a molecular marker, combining with the RNA interference technology and physiological/molecular analyses with Hipposideros armiger (a hibernating bat species) as an animal model, to explore the mechanism of Leptin in heterothermic regulation. Our data showed that all of four anti-OB-RL shRNA lentivirus significantly inhibited OB-RL expression (>90%), and the interference efficiency of PSC1742 lentivirus reached the highest value. In situ hybridization proved that PSC1742 lentivirus significantly decreased the OB-RL expression in the hypothalamus, especially in the ventromedial hypothalamic nucleus (VHM, 86.6%). Physiological analysis demonstrated that the thermoregulatory ability of bats (e.g., reducing core body temperature and heart rate) was significantly depressed after OB-RL silencing in the hypothalamus, and animals could not enter torpor state. Our study for the first time proved that the knock-down of OB-RL expression in hypothalamus inhibits heterothermic regulation of bats, and also provided the clues for further analyzing the mechanism of Leptin in the heterothermic regulation of mammals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 204, 1 August 2014, Pages 80-87
Journal: General and Comparative Endocrinology - Volume 204, 1 August 2014, Pages 80-87
نویسندگان
Tengteng Zhu, Lihong Yuan, Gareth Jones, Panyu Hua, Guimei He, Jinping Chen, Shuyi Zhang,