کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4559006 | 1628392 | 2016 | 10 صفحه PDF | دانلود رایگان |
• A metabolomics untargeted strategy was followed.
• Twelve compounds were highlighted and a classification method was developed.
• MS-based metabolomics has demonstrated its potential in food authenticity field.
The fraudulent miss-description on food product labels regarding origin or composition is a widespread problem. In this work, a metabolomic approach based on the use of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) has been applied to identify the differentiating chemical markers that allow geographic origin discrimination between different Spanish Extra Virgin Olive Oils (EVOOs). For this purpose, ninety EVOOs from 6 Spanish regions were analyzed. Data processing consisted on peak picking, retention time alignment and response normalization. Partial Least Square Discriminant Analysis (PLS-DA) and orthogonal PLS-DA (OPLS-DA) were applied to identify the most significant markers that allow groups separation. Twelve different compounds were found to correctly separate the EVOOs from their origin and 7 of them could be tentatively identified. The results of our work suggest that UHPLC-QTOF MS-based metabolomic analysis is a suitable approach for biomarker-detection in the food quality/safety field.
Journal: Food Control - Volume 70, December 2016, Pages 350–359