کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4564427 | 1628535 | 2007 | 6 صفحه PDF | دانلود رایگان |
Capillary zone electrophoresis (CZE) with photodiode array detection (PDA) was applied to simultaneously determine eleven organic acids in wine, beer, and fruit and vegetable juices after derivatisation with 2-nitrophenylhydrazine (2-NPH) in the presence of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC·HCl). The parameters affecting CZE separation included type and concentration of buffer, pH, organic additive and applied voltage. Optimum conditions at 25 °C were: 30 mmol l−1 borate buffer, pH 10.0, containing 100 ml l−1 acetonitrile, at 20 kV, sample injection at 0.5 psi for 5 s, with direct detection at 230 nm. Separation of eleven organic acids was achieved within 12 min. Linear calibration curves with good fit were obtained in the range 10.0–100.0 mg l−1. Limits of detection ranged from 2.0 to 10.0 mg l−1. Intra-day precision with RSD⩽4.0% for migration time and ⩽5.0% for peak area, and inter-day precision with RSD⩽6.0% and ⩽9.0% for migration time and peak area, were obtained. Recovery for all beverage samples was 97±15%.
Journal: LWT - Food Science and Technology - Volume 40, Issue 10, December 2007, Pages 1741–1746