کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752070 | 1415989 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Alginate-grafted Sepharose gels were characterized for cation exchangers.
- High capacity and uptake rate were achieved at ionic capacity of 240Â mmol/L.
- The grafted resin exhibited low salt tolerance in protein adsorption.
- The grafted resin kept high dynamic binding capacity at flow rate up to 1350Â cm/h.
This work was designed to acquire a new high-capacity cation-exchange chromatography resin of protein by modification of Sepharose FF gel with sodium alginate. Three cation exchangers of different ionic capacities (ICs, 140-240Â mmol/L) were synthesized, and the alginate-based resins, denoted as Alg-FFs, were studied for lysozyme (Lys) adsorption and chromatography. It was found that the adsorption capacity and effective protein diffusivity of Lys increased with increasing alginate-grafting density or the IC value. The resin with the highest IC value (240Â mmol/L), Alg-FF-240, was compared with a commercial cation exchanger, CM Sepharose FF. It revealed that both protein adsorption capacity and uptake rate of Alg-FF-240 were remarkably higher than CM Sepharose FF at NaCl concentrations lower than 100Â mmol/L, so the dynamic binding capacity (DBC) of Alg-FF-240 was much higher than CM Sepharose FF at the salt concentration range. Moreover, the DBC of Alg-FF-240 maintained high level at the flow rate up to 1350Â cm/h, and at the highest flow rate studied, the DBC of Alg-FF-240 for Lys was about 1.7 times higher than that on CM Sepharose FF. Salt-gradient elution revealed similar protein elution behaviors on the Alg-FF-240 and CM Sepharose FF columns. Altogether, the findings in this research indicate that alginate-based cation exchangers deserve further development for high-performance protein chromatography.
Journal: Biochemical Engineering Journal - Volume 126, 15 October 2017, Pages 50-57