کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752737 | 1416366 | 2017 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proline dehydrogenase-entrapped mesoporous magnetic silica nanomaterial for electrochemical biosensing of L-proline in biological fluids
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, physical adsorption was used for immobilization of proline dehydrogenase onto a magnetic mesoporous silica nanomaterial. The immobilization and electrocatalytical activity of proline dehydrogenase entrapped in a magnetic mesoporous silica nanomaterial was studied using cyclic voltammetry, differential pulse voltammetry, and square wave voltammetry. The magnetic mesoporous silica networks having a high surface area (362 m2 gâ1) exhibited excellent properties for entrapment of proline dehydrogenase. The applied approach led to better resistance to temperature and pH inactivation in comparison to the free enzyme. The electrocatalytic current response of proline dehydrogenase entrapped in a magnetic mesoporous silica nanomaterial toward oxidation of L-proline was maintained in the analytical solution temperature up to 70 °C. The entrapped proline dehydrogenase was casted onto a polycysteine-modified glassy carbon electrode. The electrode was evaluated as an electrochemical biosensor for electrooxidation and determination of L-proline in phosphate buffer solution. A cyclic voltammetry study indicated that the oxidation process of proline is irreversible and is diffusion controlled. The electrochemical behavior was further exploited as a sensitive detection scheme for L-proline determination by differential-pulse voltammetry. Under optimized conditions, the concentration range and detection limit were 0.01-0.15 μM and 0.006 μM, respectively. The method was applied to the assay of L-proline in whole blood and normal and malignant cell line lysates (normal cell (L929); gastric cancer cell-CAT 3, colon cancer cell-HCT, colon cancer cell-SW, and breast cancer cell-MCF7).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 105, October 2017, Pages 64-76
Journal: Enzyme and Microbial Technology - Volume 105, October 2017, Pages 64-76
نویسندگان
Mohammad Hasanzadeh, Arezoo Saadati Nahar, Soodabeh Hassanpour, Nasrin Shadjou, Ahad Mokhtarzadeh, Jalal Mohammadi,