کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4753073 | 1416485 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorescence and circular dichroism spectroscopy to understand the interactions between cyclodextrins and α-galactosidase from green coffee beans
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The potential of fluorescence measurement and circular dichroism spectroscopy (CDSP) to evaluate the interaction between cyclodextrins (CDs) (CD cavity size, concentration, pH, reaction time, and temperature as well as different side chain groups) and α-galactosidase was evaluated. A strong relationship was observed between α-galactosidase fluorescence intensity and CD cavity size, concentration, pH, reaction time, and temperature as well as different side chain groups. Therefore, it can be concluded that fluorescence intensity measurement can be a promising tool to ascertain β-CD-α-galactosidase interactions. CDSP is also an interesting tool to understand β-CD-α-galactosidase interactions as well as to evaluate the stability of α-galactosidase secondary structure. For instance, CDSP results showed that among three different types of CDs studied (α-CD, β-CD and γ-CD), β-CD promoted the most important α-helix transformation into β-sheet, thus reducing α-galactosidase secondary structure stability and showing the highest inhibitory effect on α-galactosidase fluorescence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Bioscience - Volume 20, December 2017, Pages 110-115
Journal: Food Bioscience - Volume 20, December 2017, Pages 110-115
نویسندگان
Wei Zou, Min Wang, Di Yao, Zhenzhou Zhu, Wei Sun, Hongyan Cai, Xuan Chen, Fang Li, Wangyang Shen, Francisco J. Barba, Wei Zhang,