کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4907569 1426571 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemically-driven benzo[a]pyrene metabolism via human cytochrome P450 1A1 with reductase coated nitrogen-doped graphene nano-composites
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Electrochemically-driven benzo[a]pyrene metabolism via human cytochrome P450 1A1 with reductase coated nitrogen-doped graphene nano-composites
چکیده انگلیسی
Benzo[a]pyrene (BaP), one of polycyclic aromatic hydrocarbons (PAHs), is identified as a prime carcinogen. Enzymatic metabolite of BaP diol epoxide is one of the important factors in its carcinogenic effect. Herein, cytochrome P450 1A1 modified nitrogen-doped graphene was synthesized to study the BaP metabolism with electrochemically-driven pathway. The direct and reversible electron transfer of the immobilized CYP1A1 on pyrenebutyric acid modified nitrogen-doped graphene nanocomposites was observed with an apparent electron transfer constant of 0.53 s− 1, and formal potential of − 0.48 V. With addition of BaP to an air-saturated buffer, the reduction peak current of dissolved oxygen increased, which confirmed the catalytic behavior of CYP1A1 to BaP. The Michaelis-Menten constant (Kmapp) and heterogeneous reaction rate constant of the catalytical reaction were calculated to be 25.6 μM and 1.9 s− 1, respectively. The inhibition effects of alpha-naphthoflavone on CYP1A1 catalyze-cycle were also illustrated with the IC50 value of 1.2 μM. The enzyme-induced BaP metabolism could be further demonstrated by the high performance liquid chromatography (HPLC) and gas chromatography-mass spectra (GC/MS). All these results showed that the proposed system had potential application for the development of drug discovery and the prediction of the chemicals toxicity in food and environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 804, 1 November 2017, Pages 23-28
نویسندگان
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