کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
49218 46724 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Synthesis of structured phosphatidylcholine containing punicic acid by the lipase-catalyzed transesterification with pomegranate seed oil
ترجمه فارسی عنوان
سنتز فسفاتیدیل کولین ساختاری حاوی پونیکیک اسید توسط ترانس اکسیداسیون لیپاز کاتالیز شده با روغن دانه انار
کلمات کلیدی
PC، فسفاتیدیل کولین؛ PSO، روغن دانه انار؛ CFA، اسید چرب کنجد؛ PUFA، اسید چرب اشباع نشده؛ CLA، اسید لینولئیک کانژوگه CLNA، اسید لینولئنی کنژوگه؛ PL، فسفولیپید؛ ALA، α-لینولنیک اسید؛ LPC، lysophosphatidylcholine؛ TAG
PC, phosphatidylcholine; PSO, pomegranate seed oil; CFA, conjugated fatty acid; PUFA, polyunsaturated fatty acid; CLA, conjugated linoleic acid; CLNA, conjugated linolenic acid; PL, phospholipids; ALA, α-linolenic acid; LPC, lysophosphatidylcholine; TAG,
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
چکیده انگلیسی


• Egg-yolk phosphatidylcholine (PC) was enzymatically enriched with punicic acid.
• Pomegranate seed oil was applied as the acyl donor.
• Among tested enzymes lipase B from Candida antarctica was the most effective.
• Optimal reaction parameters were chosen (oil:PC 3:1 M ratio and 20% enzyme load).
• 56% of punicic acid was incorporated into the sn-1 position of PC.

Incorporation of conjugated linolenic acid (punicic acid; 18:3, 9c,11t,13c) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed transesterification process was the aim of this work. Pomegranate seed oil (PSO) containing over 77% of punicic acid was used as an acyl donor and three commercially available immobilized lipases were examined as biocatalysts. The effects of enzyme load, reaction time and molar ratio of substrates (PC:PSO) on the incorporation of punicic acid into the modified PC were tested. In all experiments the best results were obtained using lipase B from Candida antarctica. In optimum conditions (20% of enzyme load; 1:3 PC:PSO molar ratio; 72 h), the incorporation of punicic acid into the sn-1 position of PC was 56%. Additionally, the total content of polyunsaturated fatty acids in modified PC was almost 50%.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Catalysis Communications - Volume 75, 5 February 2016, Pages 60–64
نویسندگان
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