کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4989059 1455976 2017 9 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Separation of xylose and glucose using an integrated membrane system for enzymatic cofactor regeneration and downstream purification
ترجمه فارسی عنوان
جداسازی زایلوز و گلوکز با استفاده از یک سیستم غشایی یکپارچه برای بازسازی و رونق کوآکتور آنزیمی و پاکسازی پس از آن
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
چکیده انگلیسی


- An integrated system for cofactor regeneration and product purification is proposed.
- High regeneration efficiency in the membrane bioreactor is obtained.
- Product purification sequence relies on size exclusion and charge repulsion.

Mixtures of xylose, glucose and pyruvate were fed to a membrane bioreactor equipped with a charged NF membrane (NTR 7450). Value-added products were obtained in the reactor via enzymatic cofactor-dependent catalysis of glucose to gluconic acid and pyruvate to lactic acid, respectively. The initial cofactor (NADH) concentration could be decreased to 10% of the stoichiometric value (relative to glucose) without compromising process time and substrate conversion via i) efficient cofactor regeneration and ii) high retention of cofactor (R=0.98) in the membrane bioreactor. Furthermore, accumulation of xylose (R<0.1), lactic acid (R=0.38) and gluconic acid (R=0.63) was minimized. After separation of the cofactor in the membrane bioreactor, xylose, lactic acid and gluconic acid were separated based on charge repulsion and size exclusion in a sequence of two NF steps. Broad substrate specificity of glucose dehydrogenase (EC 1.1.1.47) (GDH) lead to partial conversion of xylose to xylonic acid, causing some loss of xylose, but the results obtained nevertheless showed that it is possible to build a robust system for conducting enzyme reactions by sequentially regenerating the cofactor and at the same time obtaining valuable products of high purity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Membrane Science - Volume 523, 1 February 2017, Pages 327-335
نویسندگان
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