کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4996669 1459897 2018 36 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents
ترجمه فارسی عنوان
استفاده از لیپاز های تجاری غیر تجاری غیر متمرکز برای تولید بیلیت کاتالیزوری اتیل لاکتات در حلال های آلی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی
This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01-0.1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 247, January 2018, Pages 496-503
نویسندگان
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