کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4997276 | 1459905 | 2017 | 8 صفحه PDF | دانلود رایگان |
- PHP-pretreatment can promote solid loading to higher than 20% for hydrolysis.
- 73% cellulose was hydrolyzed with lower enzyme input of 20Â mg protein/g cellulose.
- Decrease on glucose yield was observed after the pretreated substrate being dried.
- Dryness shrank the large-size pores resulting in decreasing hydrolysis efficiency.
- 1Â kg wheat straw yielded 112Â g ethanol by SSF at 20% solid loading and 20Â mg/g CTec2.
Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP) for enzymatic hydrolysis and ethanol fermentation at high solid loadings. Results indicated solid loading could reach 20% with 77.4% cellulose-glucose conversion and glucose concentration of 164.9Â g/L in hydrolysate, it even was promoted to 25% with only 3.4% decrease on cellulose-glucose conversion as the pretreated-wheat straw was dewatered by air-drying. 72.9% cellulose-glucose conversion still was achieved as the minimized enzyme input of 20Â mg protein/g cellulose was employed for hydrolysis at 20% solid loading. In the corresponding conditions, 100Â g wheat straw can yield 11.2Â g ethanol with concentration of 71.2Â g/L by simultaneous saccharification and fermentation. Thus, PHP-pretreatment benefitted the glucose or ethanol yield at high solid loadings with lower enzyme input. Additionally, decreases on the maximal cellulase adsorption and the direct-orange/direct-blue indicated drying the PHP-pretreated substrates negatively affected the hydrolysis due to the shrinkage of cellulase-size-accommodable pores.
Journal: Bioresource Technology - Volume 238, August 2017, Pages 174-181