Osteogenic cell functionality on 3-dimensional nano-scaffolds with varying stiffness

To date, cellular activity on 3D substrates with stiffness ranging from a few kPa to hundreds of MPa has been investigated extensively (Cui et al., 2009; Fu et al., 2011; Hulbert et al., 1970; Hollinger et al., 1996; Johnson and Herschler, 2011; Karageorgiou and Kaplan, 2005). Fabrication limitations have restricted scaffolds with strut dimensions on the order of a few microns, a size comparable to the dimensions of osteoblasts, to have compressive moduli ranging from 10 kPa to 200 kPa, which has limited our understanding of how scaffolds stiffness affects mineral deposition. Cell viability and functionality on 3D scaffolds with compressive moduli in the MPa range and with strut dimensions on the order of a few microns have not yet been reported. We employed two-photon lithography to create periodic 3D nano-architectures with ∼99% porosity, ∼2 μm strut diameters, and ∼2-9 MPa structural stiffness to explore the influence of scaffold properties on the viability of osteoblasts in a microenvironment similar to that of natural bone. These nanolattices were made out of a polymeric core coated with different materials and had unit cells with tetrakaidecahedral geometry and a 25 μm pore size. The unit cells were tessellated in space to form a lattice with lateral dimensions of 200×200 μm and a height of 50 μm. Some of the polymer nanolattices were coated with a conformal 120 nm-thick layer of SiO2, others were coated with 120 nm of Ti. All nanolattices had a ∼20 nm-thick outermost layer of TiO2. Osteogenic cells were grown on the nanolattices for 28 days and the resulting cell morphology and depositions were characterized via scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Raman spectroscopy. These analyses revealed significant cell attachment and the presence of hydroxyapatite (Ca10(PO4)6(OH)2), tricalcium phosphate (Ca3(PO4)2) and metaphosphates ([Ca2(P2O7)]n), chemical species normally found in natural bone. Such osteogenic functionality suggests that 3-dimensional nano-architected materials can be used as effective scaffolds for cell growth and proliferation, which could eventually lead to the generation of better bone implants.