کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5130677 | 1490845 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Determination of methotrexate (MTX) in spiked blood serum using a biosensor; gold electrodes modified with antibody.
- Electrode surface modification was performed by introducing reagents while the electrodes were mounted in a flow cell.
- The electrode modification was monitored using electrochemical immittance spectroscopy and X-ray photoelectron spectroscopy.
- The method offered a wide linear working range with 8 orders of magnitude and a detection limit of 5Â Ã 10-12 mol L-1.
This article describes an attempt to develop a sensor based on multi-frequency immittance spectroscopy for the determination of methotrexate (MTX) in blood serum using gold electrodes modified with antibodies. The attachment of antibodies was monitored with electrochemical immittance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS). The EIS measurements of MTX resulted in a data matrix of size 39 Ã 55. The data were analysed using multivariate data analysis and showed a concentration dependence and time dependence that could be separated. This allowed the calculation of a multivariate calibration model. The model showed good linear behavior on a logarithmic scale offering a detection limit of 5 Ã 10â12 mol Lâ1.
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Journal: Analytica Chimica Acta - Volume 987, 22 September 2017, Pages 15-24