کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5130677 1490845 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
چکیده انگلیسی


- Determination of methotrexate (MTX) in spiked blood serum using a biosensor; gold electrodes modified with antibody.
- Electrode surface modification was performed by introducing reagents while the electrodes were mounted in a flow cell.
- The electrode modification was monitored using electrochemical immittance spectroscopy and X-ray photoelectron spectroscopy.
- The method offered a wide linear working range with 8 orders of magnitude and a detection limit of 5 × 10-12 mol L-1.

This article describes an attempt to develop a sensor based on multi-frequency immittance spectroscopy for the determination of methotrexate (MTX) in blood serum using gold electrodes modified with antibodies. The attachment of antibodies was monitored with electrochemical immittance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS). The EIS measurements of MTX resulted in a data matrix of size 39 × 55. The data were analysed using multivariate data analysis and showed a concentration dependence and time dependence that could be separated. This allowed the calculation of a multivariate calibration model. The model showed good linear behavior on a logarithmic scale offering a detection limit of 5 × 10−12 mol L−1.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 987, 22 September 2017, Pages 15-24
نویسندگان
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