کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5131061 1490882 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor
چکیده انگلیسی


- FO-SPR based competitive inhibition assay for detecting progesterone is described.
- A limit of detection of 0.5 ng mL−1 was achieved in 2-fold diluted bovine milk.
- Capacity for reducing the time-to-result was proven.
- The assay was evaluated with raw milk samples, showing excellent correlation with ELISA.

Analytical methods that are often used for the quantification of progesterone in bovine milk include immunoassays and chromatographic techniques. Depending on the selected method, the main disadvantages are the cost, time-to-result, labor intensity and usability as an automated at-line device. This paper reports for the first time on a robust and practical method to quantify small molecules, such as progesterone, in complex biological samples using an automated fiber optic surface plasmon resonance (FO-SPR) biosensor. A FO-SPR competitive inhibition assay was developed to determine biologically relevant concentrations of progesterone in bovine milk (1-10 ng/mL), after optimizing the immobilization of progesterone-bovine serum albumin (P4-BSA) conjugate, the specific detection with anti-progesterone antibody and the signal amplification with goat anti-mouse gold nanoparticles (GAM-Au NPs). The progesterone was detected in a bovine milk sample with minimal sample preparation, namely ½ dilution of the sample. Furthermore, the developed bioassay was benchmarked against a commercially available ELISA, showing excellent agreement (R2 = 0.95). Therefore, it is concluded that the automated FO-SPR platform can combine the advantages of the different existing methods for quantification of progesterone: sensitivity, accuracy, cost, time-to-result and ease-of-use.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 950, 15 January 2017, Pages 1-6
نویسندگان
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