|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5134282||1492213||2017||4 صفحه PDF||سفارش دهید||دانلود کنید|
- Stable intermediate DNA unfolding states revealed.
- Solution phase unfolding of double stranded DNA followed with ion mobility MS.
- Temperature resolved ion mobility with new temperature controlled ESI Source.
Electrospray ionization (ESI) mass spectrometry (MS) is used to study the non-covalent interactions of short 12-mer oligonucleotides under native conditions. A home-built, temperature-controlled ESI source is employed to elevate the spray temperature above the melting temperature (Tm) of the DNA duplexes, enabling the study of DNA interactions in the liquid phase rather than gas phase. Ion mobility spectrometry (IMS)-MS is used to investigate the 3-dimensional structure of the hybridized oligonucleotides and to track conformational changes in the oligonucleotide duplexes during temperature-induced melting in solution. Results show two additional drift times at TÂ >Â 35Â Â°C, indicating 2-fold (partial) unfolding dynamics for the DNA duplex with stable intermediates.
Journal: International Journal of Mass Spectrometry - Volume 419, August 2017, Pages 52-55