کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5135220 1493420 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of the stereoisomeric impurities of sitafloxacin by capillary electrophoresis with dual chiral additives
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of the stereoisomeric impurities of sitafloxacin by capillary electrophoresis with dual chiral additives
چکیده انگلیسی


- A method for determining stereoisomeric impurities of sitafloxacin was developed.
- High resolution factor was obtained by using dual chiral selectors.
- The method was optimized by using face-centred central composite design model.
- As low as 0.1% (w/w) stereoisomeric impurities can be determined.

Because of the bioactivity against the human topoisomerase II, the stereoisomeric impurities of sitafloxacin should be controlled in the process of manufactory. In the present work, a capillary electrophoresis (CE) method was developed and validated for simultaneous determination of three stereoisomeric impurities of sitafloxacin. The separation with high resolution not only for the separation of enantiomers, but also for the separation of diastereoisomers was achieved by using a background electrolyte composed of dual chiral selectors, namely γ-cyclodextrin (γ-CD) and Cu2+-d-phenylalanine (D-Phe) complex. The combination of two chiral selectors is indispensable to gain a high separation selectivity due to the cooperativity effect of different chiral discrimination modes: inclusion complexation (γ-CD) and ligand exchange (Cu2+-d-Phe). The concentrations of γ-CD, Cu2+ and D-Phe were found to be critical to the separation. Because two chiral selectors were involved in the enantiomer separation system, multiple factors and their interaction should be simultaneously optimized by using the response surface methodology (RSM) with a face centred central composite design (FCCD). The obtained optimized separation conditions were as follows: 15 mmol/L dipotassium hydrogenphosphate solution (pH 4.5) containing 15 mmol/L D-Phe, 20 mmol/L CuSO4 and 20 mmol/L γ-CD, separation voltage 15 kV. The method was then validated and the robustness of the method was tested. Under the optimized conditions, as low as 0.1% (m/m) stereoisomeric impurities of sitafloxacin can be determined by the method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1506, 14 July 2017, Pages 120-127
نویسندگان
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