کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136517 | 1494018 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A fully validated GC-TOF-MS method for the quantification of fatty acids revealed alterations in the metabolic profile of fatty acids after smoking cessation
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کلمات کلیدی
GC-TOF-MSSAFATICLOQTOFMUFAFDAm/z - m / zS/N - S / Nflame ionization detector - آشکارساز یونیزاسیون شعلهFatty acid - اسید چربsaturated fatty acid - اسید چرب اشباع شدهpoly unsaturated fatty acid - اسید چرب اشباع نشدهMono-unsaturated fatty acid - اسید چرب مونو غیر اشباعPUFA - اسید چرب چند غیراشباعSmoking cessation - ترک سیگارLOD یا Limit of detection - حد تشخیصFID - درtime of flight - زمان پروازSIM - سیم کارتCoefficient of variance - ضریب واریانسmass spectrometer - طیف سنج جرمیinner diameter - قطر داخلیMetabolomics - متابولومیکMethyl ester - متیل استرFatty acid methyl ester - متیل استر اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربlimit of quantification - محدودیت اندازه گیریlimit of detection - محدودیت تشخیصFatty acid profile - مشخصات اسید چربMass-to-charge ratio - نسبت جرم به شارژsignal to noise ratio - نسبت سیگنال به نویزselected ion monitoring - نظارت بر یون انتخاب شدهMetabolic profiling - پروفیل متابولیکHuman plasma - پلاسمای انسانیgas chromatograph - کروماتوگرافی گازtotal ion chromatogram - کروماتوگرافی یونی کلquality control - کنترل کیفیتelectron impact ionization - یونیزاسیون اثر الکترون
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We developed and validated an efficient and robust method for the simultaneous quantification of 44 fatty acid species in human plasma via GC-TOF-MS. The method is characterized by its robustness, accuracy and precision covering a wide range of fatty acid species with various saturation degrees including short chain fatty acids (beginning with FA 4:0) and long chain fatty acids (up to FA 32:0). The fatty acids were methylated prior to analyses and subsequently detected as fatty acid methyl esters by means of GC-TOF-MS. A highly substituted polar column allowed the separation of geometrical and positional isomers of fatty acid species. The method was applied to plasma samples of a strictly diet controlled clinical smoking cessation study including 39 smokers followed over the course of three months after having quit. Statistical significant alterations within the fatty acid profile were observed when comparing the baseline (subjects still smoking) with one week, one month and three months of smoking cessation. After 3 months of smoking cessation, a partial recovery of alterations in the fatty acid profile evoked by smoking was observed. In conclusion, the developed fatty acid profiling method using GC-TOF-MS has proven as a reliable tool for the quantitative determination of 44 individual fatty acid species within clinical studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1041â1042, 15 January 2017, Pages 141-150
Journal: Journal of Chromatography B - Volumes 1041â1042, 15 January 2017, Pages 141-150
نویسندگان
Michael Goettel, Reinhard Niessner, Nikola Pluym, Gerhard Scherer, Max Scherer,