کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136840 | 1494481 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Quali-quantitative analysis of glucosinolates in Raphanus raphanistrum tissues.
- MRM method was validated according to ICH.
- PCA highlighted similarities/differences among tissues of R. raphanistrum.
- The obtained data have commercial value in the field of food/medicinal plant.
This study aims to identify and quantify the glucosinolates from different parts of wild radish R. raphanistrum (leaves, flowers, fruits, roots) using liquid chromatography-tandem mass spectrometry. Glucoraphenin is the predominant compound, accounting for about 87% (w/w) of total glucosinolate content, followed by glucobrassicin, glucoraphasatin and glucoraphanin (153Â mg 100Â gâ1, 149Â mg 100Â gâ1 and 141Â mg 100Â gâ1 FW, respectively) in fruits; followed by glucoraphasatin (3Â mg 100Â gâ1 FW) in flowers and by glucobrassicin, 4-hydroxyglucobrassicin and glucoraphasatin (145Â mg 100Â gâ1, 27Â mg 100Â gâ1 and 24Â mg 100Â gâ1 FW, respectively) in leaves. In roots the major glucosinolate is glucoraphasatin (56Â mg 100Â gâ1 FW) followed by the glucoraphenin and methoxyglucobrassicin (16Â mg 100Â gâ1 and 7Â mg 100Â gâ1 FW, respectively). Principal component analysis allowed the discrimination of fruit samples from other parts of the plant for the majority of glucosinolates and the fruits are highlighted as sources of glucosinolates. The results are interesting given that wild radish is one of the most important weeds of crops in the Mediterranean region and is popular for home vegetable production and for its employment in human nutrition both as a food as well as for medicinal purposes.
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Journal: Journal of Food Composition and Analysis - Volume 61, August 2017, Pages 20-27