کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5137713 1494587 2018 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A sensitive HPLC-MS method for simultaneous determination of thirteen components in rat plasma and its application to pharmacokinetic study of Tanreqing injection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A sensitive HPLC-MS method for simultaneous determination of thirteen components in rat plasma and its application to pharmacokinetic study of Tanreqing injection
چکیده انگلیسی
A rapid, sensitive and selective method based on high-performance liquid chromatography coupled with Q-Exactive mass spectrometry was developed and validated for simultaneous quantification of thirteen components in rat plasma, including chlorogenic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid, luteoloside, isochlorogenic acid A, B and C, scutellarin, baicalin, wogonin, baicalein, phillyrin and forsythoside A. After precipitating proteins from the plasma samples with methanol, chromatographic separation of the thirteen components was achieved by using an XBridge™ C18 column (2.1 mm × 150 mm, 5 μm) with the mobile phase consisting of methanol and 0.1% formic acid in water at a flow rate of 0.3 mL/min. High-resolution MS quantification was adopted with detection on a Q-Exactive mass spectrometer in full-scan mode, and the results were obtained using a mass extraction window of 10 ppm at a mass resolution of 70, 000. All the calibration curves exhibited good linearity (r2 > 0.991) over the measured ranges. The lower limit of quantitation (LLOQ) was in the range of 1.05-8.13 ng/mL. The intra- and inter-day precision (RSD) was less than 11.70% and the accuracy (RE) ranged from −5.58% to 12.29%. No significant matrix effect was observed and the extraction recoveries of all the analytes were more than 79.36%. The developed method was applied to a pharmacokinetic study of the thirteen ingredients in rats after intravenous administration of Tanreqing at three doses of 3, 6 and 12 mL/kg. The results indicated that 8 of the 13 components, isochlorogenic acid A, B and C, chlorogenic acid, baicalin, wogonin, luteoloside and forsythoside A, had linear pharmacokinetic properties in the tested dosage range.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 148, 30 January 2018, Pages 205-213
نویسندگان
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