کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5156389 | 1500579 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A gene encoding a novel dextransucrase was identified in the genome of Oenococcus kitaharae DSM17330 and cloned into E. coli. With a kcat of 691 sâ1 and a half-life time of 111 h at 30 °C, the resulting recombinant enzyme -named DSR-OK- stands as one of the most efficient and stable dextransucrase characterized to date. From sucrose, this enzyme catalyzes the synthesis of a quasi linear dextran with a molar mass higher than 1 Ã 109 g·molâ1 that presents uncommon rheological properties such as a higher viscosity than that of the most industrially used dextran from L. mesenteroides NRRL-B-512F, a yield stress that was never described before for any type of dextran, as well as a gel-like structure. All these properties open the way to a vast array of new applications in health, food/feed, bulk or fine chemicals fields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 179, 1 January 2018, Pages 10-18
Journal: Carbohydrate Polymers - Volume 179, 1 January 2018, Pages 10-18
نویسندگان
Marlène Vuillemin, Florent Grimaud, Marion Claverie, Agnès Rolland-Sabaté, Catherine Garnier, Patrick Lucas, Pierre Monsan, Marguerite Dols-Lafargue, Magali Remaud-Siméon, Claire Moulis,