Very simple combination of TROSY, CRINEPT and multiple quantum coherence for signal enhancement in an HN(CO)CA experiment for large proteins

► 3D TROSY-MQ/CRINEPT-HN(CO)CA is a high sensitive NMR triple resonance experiment for sequential assignment. ► A signal enhancement of a factor of ∼1.8 is obtained on the membrane protein KcsA when compared with a conventional TROSY-based HN(CO)CA experiment. ► A similar signal enhancement is observed for the small model protein ubiquitin highlighting the importance of less soft pulses required.