کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505391 | 1400267 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
WNT/β-catenin pathway modulates the TNF-α-induced inflammatory response in bronchial epithelial cells
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کلمات کلیدی
TNF-RfrizzledMCPTBPLRPDVLTNFGSKWntinflammation - التهاب( توروم) interleukin - اینترلوکینβ-catenin - بتا-کاتنینdisheveled - بی رحمCOPD - بیماری مزمن انسدادی ریهChronic obstructive pulmonary disease - بیماری مزمن انسدادی ریهsmall interfering - دخالت کوچکBronchial epithelial cell - سلول اپیتلیال برونشtumor necrosis factor - فاکتور نکروز تومورTNF-α - فاکتور نکروز توموری آلفاpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازTATA box binding protein - پروتئین اتصال دهنده جعبه TATAmonocyte chemoattractant protein - پروتئین شیمیایی monocyte chemoattractantLipoprotein receptor-related protein - پروتئین مرتبط با گیرنده لیپوپروتئینglycogen synthase kinase - گلیکوزین سیتستاز کینازtumor necrosis factor receptor - گیرنده فاکتور نکروز تومور
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, TNF-α was found to activate the WNT/β-catenin pathway in BEAS-2B human bronchial epithelial cells. Levels of phospho-LRP6, Dvl-2, and phospho-GSK-3β were elevated, while that of Axin was reduced by TNF-α treatment. Nuclear translocation of β-catenin and the reporter activity of a β-catenin-responsive promoter were increased by TNF-α treatment. Under the same experimental conditions, TNF-α activated the NF-κB signaling, which includes the phosphorylation and degradation of IκB and nuclear translocation and target DNA binding of NF-κB, and it was found that an inhibitor of NF-κB activation, JSH-23, inhibited TNF-α-induced Wnt signaling as well as NF-κB signaling. It was also found that recombinant Wnt proteins induced NF-κB nuclear translocations and its target DNA binding, suggesting that Wnt signaling and NF-κB signaling were inter-connected. TNF-α-induced modulations of IκB and NF-κB as well as pro-inflammatory cytokine expression were significantly suppressed by the transfection of β-catenin siRNA compared to that of control siRNA. Transfection of a β-catenin expression plasmid augmented the TNF-α-induced modulations of IκB and NF-κB as well as pro-inflammatory cytokine expression. These results clearly demonstrated that the WNT/β-catenin pathway modulates the inflammatory response induced by TNF-α, suggesting that this pathway may be a useful target for the effective treatment of bronchial inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 484, Issue 2, 4 March 2017, Pages 442-449
Journal: Biochemical and Biophysical Research Communications - Volume 484, Issue 2, 4 March 2017, Pages 442-449
نویسندگان
Jaewoong Jang, Yoonju Jung, Seyeon Chae, Sang-In Chung, Seok-Min Kim, Yoosik Yoon,