کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5506098 | 1400285 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel transmembrane protein defines the endoplasmic reticulum stress-induced cell death pathway
ترجمه فارسی عنوان
پروتئین ترمومبنال جدید مسیر مرگ سلولی ناشی از استرس سلول اندوپلاسمی را تعیین می کند
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Mitochondrial membrane potential (ÎΨm) maintenance is physiologically critical in cells; its loss causes apoptotic signalling and cell death. Accumulating DNA mutations and unfolded proteins in stressed cells activate signalling pathways for cell death induction. Cancer cells often fail to die even in the presence of some death signalling proteins. Here, we report a short hairpin RNA (shRNA) with an artificial sequence, denoted Psi1 shRNA, which leads to ÎΨm loss in HCT116 cells. The Psi1 shRNA target gene was shown to encode transmembrane protein 117 (TMEM117). TMEM117 knockdown led to ÎΨm loss, increased reactive oxygen species levels, up-regulation of an endoplasmic reticulum (ER) stress sensor C/EBP homologous protein and active caspase-3 expression, and cell growth impairment, altering homeostasis towards cell death. TMEM117 levels were down-regulated in response to the ER stressor thapsigargin and decreased when cells showed ÎΨm loss. These results suggested that TMEM117 RNAi allowed apoptotic cell death. Therefore, TMEM117 probably mediates the signalling of ÎΨm loss in ER stress-mediated mitochondria-mediated cell death.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 486, Issue 1, 22 April 2017, Pages 149-155
Journal: Biochemical and Biophysical Research Communications - Volume 486, Issue 1, 22 April 2017, Pages 149-155
نویسندگان
Tomoya Tamaki, Kenta Kamatsuka, Taku Sato, Shuntaro Morooka, Kosuke Otsuka, Masahiro Hattori, Tomoyasu Sugiyama,