کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510512 | 1539131 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and characterization of unfolded protein response genes from large yellow croaker (Larimichthys crocea) and their expression in response to dietary fatty acids
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کلمات کلیدی
ATF4NF-κBERADLC-PUFAER-associated protein degradationeIF2αATF6BiP/Grp78 - BIP / Grp78C/EBP homologous protein - C / EBP پروتئین همولوگIRE1α - IRE1ainterleukin - اینترلوکینCHOP - تکه کردنOlive oil - روغن زیتونFish oil - روغن ماهیPerilla oil - روغن پرایلاendoplasmic reticulum - شبکه آندوپلاسمی activating transcription factor 4 - فعال کردن عامل رونویسی 4activating transcription factor-6 - فعال کردن عامل رونویسی 6PERK - پرک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The unfolded protein response (UPR) is a mechanism to cope with perturbed endoplasmic reticulum (ER) functions or accumulation of unfolded protein in the ER in eukaryotic cells. Furthermore, the UPR also participates in a number of physiological and pathological processes, such as nutrient sensing, lipid synthesis, and inflammatory response. In this study, four UPR-related genes (GRP78/BiP, ATF6α, XBP1 and CHO) were isolated characterized from large yellow croaker (Larimichthys crocea), and their expression in response to dietary lipid sources (various fatty acids) such as fish oil (FO), palmic acid (PA), olive oil (OO), sunflower oil (SO), and perilla oil (PO), were examined following feeding. The results showed that the four UPR-related proteins contained highly conserved functional domains and had the closest phylogenetic relationships with other fishes. Additionally, these genes were ubiquitously expressed in large yellow croaker, as in zebrafish and medaka. Moreover, GRP78, ATF6α and spliced XBP1 (XBP1s) mRNA levels in the liver, not in adipose tissue, were significantly increased in the SO group compared to the other groups (P < 0.05). These results indicated that dietary SO activated UPR, and the activation of UPR might provide a mechanism to improve ER function, but probably stimulated lipid synthesis and caused inflammatory response in the liver of large yellow croaker.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 203, January 2017, Pages 53-64
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 203, January 2017, Pages 53-64
نویسندگان
Kai Liao, Jing Yan, Songlin Li, Tianjiao Wang, Wei Xu, Kangsen Mai, Qinghui Ai,