کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5516967 1543035 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of a broad-specificity monoclonal antibody and application as a receptor to detection amatoxins in mushroom
ترجمه فارسی عنوان
تولید یک آنتی بادی منوکلونال وسیع اختصاصی و کاربرد آن به عنوان گیرنده برای تشخیص آماتوکسین ها در قارچ
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی

In this study, we report the production of a monoclonal broad-specificity monoclonal antibody (mAb) specific for amatoxins and development of an indirect competitive immunoassay for detection of amatoxins in mushroom samples. In the assay, the complete antigen (α-amanitin-OVA) was used as coating antigen, and amatoxins as competitor competes with coating antigen to bind with mAb. Using this approach, The half-maximum inhibition concentrations (IC50) of α-amanitin, β-amanitin and γ-amanitin, and limits of detection (LODs, IC15) were 66.3, 97.4, 163.1 ng/mL and 0.91, 0.98, 0.89 ng/mL, respectively. The LODs for α-amanitin, β-amanitin and γ-amanitin in mushroom samples were 4.55, 4.9, and 4.45 ng/mL. The spiked results were also confirmed by HPLC, which showed a good correlation (R2 = 0.996) between the two methods. The results indicated that the developed assay was reliable and suitable for the detection of amatoxins in mushroom samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 49, September 2017, Pages 57-61
نویسندگان
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