کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5521424 1545305 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperInfluence of different stabilizers on the encapsulation of desmopressin acetate into PLGA nanoparticles
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Research paperInfluence of different stabilizers on the encapsulation of desmopressin acetate into PLGA nanoparticles
چکیده انگلیسی

To address targeting and bioavailability issues of peptidic drugs like desmopressin, the encapsulation into nanoparticles (NP) has become standard in pharmaceutics. This study investigated the encapsulation of desmopressin into PLGA NP by the use of pharmaceutically common stabilizers as a precursor to future, optional targeting and bioavailability experiments. Polymer dry weights were measured by freeze drying and thermo gravimetric analysis (TGA). Particle sizes (ranging between 105 and 130 nm, PDI < 0.1) and zeta potentials (−35 to −45 mV) were analyzed with Dynamic Light Scattering (DLS) and Laser-Doppler-Anemometry (LDA) respectively. Highest loading efficiencies, quantified by RP-HPLC, were achieved with Pluronic F-68 as stabilizer of the inner aqueous phase (1.16 ± 0.07 μg desmopressin/mg PLGA) and were significantly higher than coating approaches and approaches without stabilizer (0.74 ± 0.01 μg/mg). Optimized nanoformulations are thus in competition with the concentration of commercial non-nanoparticulate desmopressin products. Stability of desmopressin after the process was evaluated by HPLC peak purity analysis (diode array detector) and by mass spectrometry. Desmopressin was shown to remain intact during the whole process; however, despite these very good results the encapsulation efficiency turned out to be a bottle neck and makes the system a challenge for potential applications.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 118, September 2017, Pages 48-55
نویسندگان
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