کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5561326 | 1562116 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of autophagy aggravated 4-nitrophenol-induced oxidative stress and apoptosis in NHPrE1 human normal prostate epithelial progenitor cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EGFDMEMFBS3-MAITSmTORPNPLC3BPE3-methyladenine - 3-متیل آدنین4-Nitrophenol - 4-نیتروفنولDulbecco's modified Eagles medium - Medal of Eagles اصلاح شده DulbeccoAutophagy - اتوفاژیOxidative stress - تنش اکسیداتیوApoptosis - خزان یاختهایstandard error of the mean - خطای استاندارد میانگینlight chain 3 - زنجیره سبک 3fetal bovine serum - سرم جنین گاوepidermal growth factor - عامل رشد اپیدرمیbovine pituitary extract - عصاره هیپوفیز گاوSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیmammalian target of rapamycin - هدف پستانداران رپامایسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
4-Nitrophenol (PNP), a well-established human carcinogen, has been proven to have detrimental effects on reproductive system of male rats in previous studies. The molecular mechanisms involved PNP-induced damage remain to be established. Autophagy can exert protective effects on various cytotoxic factors that induce injury. In the present study, we aim to investigate whether autophagy is induced by PNP and the function of autophagy in PNP-induced injury in NHPrE1, a normal human prostate epithelial progenitor cell line. Our results indicate that PNP induced oxidative stress as evidenced by increased MDA levels and decreased activity of SOD and GSH-Px. PNP also increased apoptosis of NHPrE1 cells as evidenced by western blot and Hoechst 33258 staining and activated autophagy in NHPrE1 cells detected by RT-PCR and western blot. Inhibition of autophagy by 3-MA further increased PNP-induced oxidative stress and apoptosis of NHPrE1 cells. We also found that PNP-induced apoptosis was suppressed by N-acetylcysteine, suggesting oxidative stress may play an important role in PNP cytotoxicity. Furthermore, phosphorylation of mTOR protein was inhibited by PNP, indicating that PNP might induce autophagy in NHPrE1 cells via inhibiting mTOR pathway. In conclusion, these results suggest that activation of autophagy should play a protective role in PNP-induced oxidative stress and apoptosis of NHPrE1 cells, which might be mediated through mTOR pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 87, July 2017, Pages 88-94
Journal: Regulatory Toxicology and Pharmacology - Volume 87, July 2017, Pages 88-94
نویسندگان
Yonghui Zhang, Chong Zhang, Fulu Dong, Miaomiao Chen, Jingchen Cao, Haiyan Wang, Ming Jiang,