کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5849533 1561760 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The development and validation of methods for evaluating the immune system in preweaning piglets
ترجمه فارسی عنوان
توسعه و اعتبار سنجی روش های ارزیابی سیستم ایمنی در پیش آلوئه ورا
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Three different assays were developed and validated to assess piglet immune system.
- GLP validation followed a fit for purpose approach to establish parameters and acceptance criteria.
- All three assays passed acceptance criteria and were accepted for use on GLP studies.

The preweaning piglet has been found to be a valuable research model for testing ingredients used in infant formula. As part of the safety assessment, the neonates' immune system is an important component that has to be evaluated. In this study three concurrent strategies were developed to assess immune system status. The methods included (1) immunophenotying to assess circulating innate immune cell populations, (2) monitoring of circulating cytokines, particularly in response to a positive control agent, and (3) monitoring of localized gastrointestinal tissue cytokines using immunohistochemistry (IHC), particularly in response to a positive control agent. All assays were validated using white papers and regulatory guidance within a GLP environment. To validate the assays precision, accuracy and sample stability were evaluated as needed using a fit for purpose approach. In addition animals were treated with proinflammtory substances to detect a positive versus negative signal. In conclusion, these three methods were confirmed to be robust assays to evaluate the immune system and GIT-specific immune responses of preweaning piglets.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 84, October 2015, Pages 197-207
نویسندگان
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