کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905161 | 1159854 | 2016 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and functional analysis of BdFoxO gene in the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae)
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کلمات کلیدی
RT-qPCRINRdsRNA4E binding protein4EBPBactrocera dorsalisPDKILPsTORJnkPBSPI3KJun NH2-terminal kinaseRNA interference - RNA تداخل کنندهdouble-stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهAkt - آکتforkhead Box O - جعبه O جعبه OFoxO - فاکسوPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهtarget of rapamycin - هدف از رپامایسینReverse Transcription-quantitative Polymerase Chain Reaction - واکنش زنجیره ای پلیمراز کمی رونویسی معکوسprotein kinase B - پروتئین کیناز BExpression profiles - پروفایل های بیانInsulin-like peptides - پپتیدهای انسولین مانندphosphoinositide-dependent kinase - کیناز وابسته به فسفوئینوزیتهStarvation - گرسنگیinsulin receptor - گیرنده انسولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The forkhead box O transcription factor (FoxO) is an important downstream transcription factor in the well-conserved insulin signaling pathway, which regulates the body size and development of insects. In this study, the FoxO gene (BdFoxO) was identified from the oriental fruit fly, Bactrocera dorsalis (Hendel). The open reading frame of BdFoxO (2732Â bp) encoded a 910 amino acid protein, and the sequence was well conserved with other insect species. The BdFoxO was highly expressed in larvae and pupae among different development stages, and the highest tissue-specific expression level was found in the fat bodies compared to the testis, ovary, head, thorax, midgut, and Malpighian tubules of adults. Interestingly, we found BdFoxO expression was also up-regulated by starvation, but down-regulated when re-fed. Moreover, the injection of BdFoxO double-stranded RNAs into third-instar larvae significantly reduced BdFoxO transcript levels, which in turn down-regulated the expression of other four genes in the insulin signaling pathway. The silencing of BdFoxO resulted in delayed pupation, and the insect body weight increased significantly compared with that of the control. These results suggested that BdFoxO plays an important role in body size and development in B. dorsalis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 578, Issue 2, 10 March 2016, Pages 219-224
Journal: Gene - Volume 578, Issue 2, 10 March 2016, Pages 219-224
نویسندگان
Yi-Bei Wu, Wen-Jia Yang, Yi-Fei Xie, Kang-Kang Xu, Yi Tian, Guo-Rui Yuan, Jin-Jun Wang,